Two simple, sensitive and accurate spectrophotometric methods for the determination of pantoprazole sodium have been developed and validated. The methods are based on oxidation of pantoprazole sodium with ferric ammonium sulphate followed by complex formation of resulting ferrous ion (Fe2+) with 1, 10-phenanthroline to form orange red coloured chromogen (method A), while pantoprazole sodium in presence of acidic medium reacts with excess amount of chloramine-T and the unreacted chloramine-T reacts with crystal violet to produce greenish blue coloured chromogen (method B). The final stock solution was made to produce 100 µg/ml with distilled water for both methods A and method B respectively. The λmax was found to be 507 nm and 620 nm for method A and method B respectively. The linearity was found in concentration range of 10-50 µg/ml and 5-30 µg/ml respectively, for method A and method B. The correlation coefficient was found 0.9986 and 0.9991 for method A and method B respectively. The regression equation was found as Y = 0.0198x + 0.0202 and Y = 0.0251x + 0.0022 respectively for method A and method B. The methods were validated according to ICH and USP guidelines.
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